Top 100 USA AI Startups to Watch in 2025

The artificial intelligence landscape in 2025 isn't just growing; it's undergoing a Cambrian explosion of innovation. From next-generation defense systems and AI-powered drug discovery to autonomous agents automating entire business workflows, the pace of change is staggering. Keeping track of the key players in this dynamic ecosystem has become a challenge for investors, engineers, and industry leaders alike.

That's why we've compiled this mega-directory of 100 of the most legitimate and promising AI startups in the USA. This curated list cuts through the noise, featuring everything from stealth-mode Y Combinator graduates and university spin-offs to established unicorns transforming their industries. We cover the entire spectrum: enterprise SaaS, healthcare, robotics, generative AI, developer tools, and the ambitious labs chasing Artificial General Intelligence (AGI).

This is more than just a list; it's a map to the future. Whether you're an investor seeking the next unicorn, a talented professional looking for your next challenge, or a business leader trying to understand the coming wave of disruption, this directory is your essential guide. Let's dive in.

The 2025 AI Startup Landscape at a Glance

Before diving into the full list, this table highlights the dominant trends and sectors represented by our AI 100. It shows a clear shift from general-purpose tools to highly specialized, workflow-native AI agents solving critical, real-world problems.

Key Trend / Sector	Core Focus	Why It Matters in 2025	Example Companies from the List
Healthcare & Life Sciences 🧬	Using AI for drug discovery, diagnostics, and reducing administrative burden.	AI is dramatically accelerating clinical trials and personalizing patient care, creating massive value and saving lives.	PathAI, Insitro, Anterior, Delfina
Enterprise & Data Intelligence📈	Building AI co-pilots and agents to automate workflows, analyze data, and enhance productivity.	AI is becoming the new operating system for business, moving from a "nice-to-have" tool to essential infrastructure.	Databricks, Glean, Writer, Scale AI
Defense & Autonomous Systems 🤖	Developing intelligent drones, vehicles, and software for national security and logistics.	Autonomy is the next frontier in defense and supply chain, promising safer and more efficient operations at an unprecedented scale.	Anduril, Shield AI, Waymo, Zipline
The Generative Wave 🎨	Creating new content, from hyper-realistic video and art to SEO-optimized text and code.	Generative models are democratizing creativity and content production, creating entirely new markets and professions.	Midjourney, Synthesia, OpenAI, Runway
The Emerging Vanguard🚀	Building "workflow-native" AI agents that invisibly solve high-value problems in specific industries.	This new wave represents the maturation of AI—it's less about the tech itself and more about the tangible business problems it can solve.	Mandel AI, Contour, Harmonic, XBOW

The Top 100 USA AI Startups in 2025: The Definitive Directory

1. Anysphere

   Bio: A rapidly growing AI startup founded in 2022, Anysphere is known for its influential coding tool, Cursor. The company empowers engineers to write and edit code using natural language, serving major clients like Instacart and generating significant revenue.
   

   Website: https://www.cursor.com/

   Key Product: Cursor is an AI-powered code editor and assistant designed to streamline the software development workflow.
   

2. Mercor

   Bio: Founded in 2023 and already valued at $2 billion, Mercor is an AI startup that automates the hiring process. It uses artificial intelligence to screen résumés and cover letters and conducts avatar-based video interviews to reduce bias and increase efficiency. Website: https://mercor.com/

   Key Product: Mercor offers an AI-driven employment screening platform that automates and standardizes the initial phases of talent acquisition.
   

3. World Labs

   Bio: Launched in 2024 by renowned AI pioneer Fei-Fei Li, World Labs is a startup dedicated to creating AI models that can understand and generate physical environments. Its technology is aimed at applications in advanced simulations and training.
   

   Website: https://worldlabs.ai/

   Key Product: The company develops spatial intelligence AI models capable of creating and interacting with realistic virtual environments for simulation and training purposes.
   

4. OpenEvidence

   Bio: A unicorn startup operating in the healthcare sector, OpenEvidence builds AI-powered tools to help medical professionals quickly make sense of vast amounts of medical literature and clinical data.

   Website: https://openevidence.com/

   Key Product: The OpenEvidence platform is an AI-powered medical search and summarization tool that provides healthcare professionals with rapid, evidence-based insights.
   

5. Thinking Machine Labs

   Bio: Founded in 2025 by former OpenAI CTO Mira Murati, Thinking Machine Labs is a stealth startup that has raised $1 billion to develop advanced, broadly capable artificial intelligence systems.

   Website: Not publicly available (stealth mode)

   Key Product: The company is focused on creating next-generation, general-purpose AI systems.
   

6. Writer

   Bio: Writer is an enterprise-focused AI company that specializes in training custom large language models tailored for specific business needs, such as content creation, document analysis, and maintaining brand consistency.

   Website: https://writer.com/

   Key Product: Writer provides a full-stack generative AI platform that helps businesses build custom models for tasks like marketing content creation and internal document search.
   

7. Crusoe

   Bio: Valued at $2.8 billion, Crusoe is an AI infrastructure provider that builds energy-efficient data centers and cloud infrastructure. The company focuses on powering compute-intensive AI workloads in an environmentally friendly manner.
   

   Website: https://crusoe.ai/

   Key Product: Crusoe offers clean cloud infrastructure, including GPUs and data centers, specifically designed for demanding AI and machine learning tasks.
   

8. Lambda

   Bio: Lambda is an AI infrastructure firm valued at $2.5 billion, providing the essential hardware for training large-scale AI models. It offers on-demand access to powerful GPUs and fully configured data center solutions.
   

   Website: https://lambdalabs.com/

   Key Product: Lambda provides an on-demand GPU cloud service and dedicated hardware for AI developers and researchers needing high-performance computing resources.
   

9. Together AI

   Bio: Valued at $3.3 billion, Together AI provides a cloud platform designed to make it easier for developers to train, fine-tune, and deploy open-source artificial intelligence models with scalable and efficient infrastructure.
   

   Website: https://www.together.ai/

   Key Product: The Together AI Cloud is an AI acceleration platform offering serverless APIs and scalable infrastructure for running and customizing open-source models.
   

10. Frame AI

    Bio: Frame AI is a New York-based company that builds AI software to analyze customer communication data from various sources. It helps businesses detect patterns, identify risks, and convert customer feedback into actionable insights to improve retention.
    

    Website: https://frame.ai/

    Key Product: The company's Voice of the Customer engine uses AI to consolidate and analyze customer feedback, providing a unified view of the customer experience.
    

11. Moveworks

    Bio: Moveworks provides an AI platform designed to automate workplace support. It uses conversational AI to understand and resolve employee issues in real-time, such as IT troubleshooting, HR requests, and facilities management.
    

    Website: https://www.moveworks.com/

    Key Product: The Moveworks platform is an advanced employee support chatbot that integrates with enterprise systems to provide instant, autonomous assistance.
    

12. Codeium

    Bio: Codeium offers an AI-powered toolkit for software developers. Its platform provides real-time code suggestions, intelligent code search, and a chatbot assistant to help developers write code faster and more efficiently.
    

    Website: https://codeium.com/

    Key Product: Codeium Chat and IDE integrations provide a comprehensive AI coding assistant that supports developers throughout the software lifecycle.
    

13. Soundful

    Bio: Soundful is an AI platform that enables creators to generate customizable, royalty-free soundtracks for their content. Users can create unique music for videos, podcasts, and other digital media based on mood, genre, and context.
    

    Website: https://soundful.com/

    Key Product: The Soundful music generation platform uses AI to create high-quality, personalized soundtracks on demand.
    

14. Dialpad

    Bio: Dialpad is a customer intelligence platform that embeds AI into business communications. Its services for calls, meetings, and messaging provide real-time transcriptions, sentiment analysis, and automated summaries to enhance sales and customer engagement.
    

    Website: https://www.dialpad.com/

    Key Product: Dialpad offers a suite of AI-powered communication tools that unify voice, video, and messaging with real-time analytics and automation.

    
    

15. Yellow.ai Bio: Yellow.ai provides a conversational AI platform for automating customer service and employee support. It enables businesses to build and deploy intelligent chatbots and voice bots in over 100 languages across more than 35 channels. Website: https://yellow.ai/ Key Product: The Dynamic Automation Platform (DAP) allows enterprises to create sophisticated AI agents for customer experience, employee experience, and marketing automation.
    

16. PlayHT Bio: PlayHT is an AI-driven text-to-speech platform that generates highly realistic, human-like voices. It is used for creating voiceovers, podcasts, e-learning materials, and other audio content in over 142 languages and accents. Website: https://play.ht/ Key Product: PlayHT offers an advanced AI text-to-speech converter that produces natural-sounding voice performances from text input.
    

17. MindsDB Bio: MindsDB is an open-source AutoML platform that simplifies the process of building and deploying predictive models. It allows developers to use standard SQL queries to create and utilize machine learning models directly within their databases. Website: https://mindsdb.com/ Key Product:MindsDB's core offering is in-database machine learning, which brings predictive capabilities to existing data infrastructure without requiring specialized expertise.
    

18. Shield AI Bio: Shield AI is a defense technology company focused on developing autonomous systems for military applications. Its AI pilot, Hivemind, enables drones and aircraft to operate autonomously in high-threat environments for reconnaissance and command tasks. Website: https://shield.ai/ Key Product: Hivemind is an AI pilot that can be integrated into various aircraft, enabling autonomous maneuvering, coordination, and execution of missions.
    

19. Eightfold Bio: Eightfold provides an AI-powered platform for human resources, specifically focused on talent management. It helps large enterprises like Capital One with talent acquisition, retention, and employee development by analyzing skills and career paths. Website: https://eightfold.ai/ Key Product:The Talent Intelligence Platform uses a deep-learning AI to help companies manage the entire talent lifecycle, from hiring to workforce planning.
    

20. Inflection AI Bio: Inflection AI is a prominent AI lab that pivoted from its consumer-facing chatbot, Pi, to focus on building custom large language models and APIs for enterprise clients. The company specializes in creating customizable generative AI for businesses. Website: https://www.inflection.ai/ Key Product: Inflection offers services for creating custom LLMs and conversational agents tailored to specific business needs and workflows.
    

21. Cognition AI Bio: Cognition AI is the creator of Devin, the world's first autonomous AI software engineer. The company is focused on building AI agents capable of handling complex, end-to-end software development tasks, from planning to writing and debugging code. Website:https://www.cognition.ai/ Key Product: Devin is an autonomous AI coding assistant designed to perform complete software engineering projects with minimal human intervention.
    

22. Celestial AI Bio: Celestial AI is a hardware startup that creates photonic interconnects for AI infrastructure. Its technology addresses the memory-compute bottleneck by enabling high-bandwidth, low-latency data transfer at scale, which is critical for advanced AI systems. Website:https://www.celestial.ai/ Key Product: The Photonic Fabric is an optical interconnect platform that provides a faster and more efficient link between compute and memory for AI data centers.
    

23. Pathos Bio: Pathos is a biotechnology firm that leverages multimodal AI to accelerate oncology drug development. By integrating diverse biological data, its platform aims to identify new therapeutic targets and optimize clinical trials for cancer treatments. Website: https://www.pathos.ai/ Key Product: PathOS is the company's AI foundation model for cancer research, designed to provide novel insights into tumor biology and patient responses.

    
    

24. Magic Bio: Magic is an AI startup dedicated to building foundation models for programming. Its goal is to create AI "colleagues" that can assist with complex code writing, planning, and reasoning tasks, going beyond simple code completion. Website: https://www.magic.dev/ Key Product: The company is developing Long-Term Memory (LTM) networks to give its AI coding agents a deep understanding of large codebases and complex contexts.
    

25. Skild AI Bio: Skild AI is a robotics startup developing a general-purpose AI "brain" for robots. Its foundation model allows robots to perform a wide variety of tasks without needing to be specifically programmed for each one, enabling greater versatility. Website: https://www.skild.ai/ Key Product:Skild AI is building a robotics foundation model that provides a unified control system for different types of robots across various tasks.
    

26. Decagon Bio: Decagon develops autonomous AI agents designed to automate customer support operations. Its platform can handle customer inquiries across chat, email, and voice channels by integrating with a company's existing enterprise systems. Website: https://www.decagon.ai/ Key Product: Decagon offers omnichannel AI support agents that can autonomously resolve customer issues with human-like conversational abilities.
    

27. Anthropic Bio: Founded in 2021 by former OpenAI researchers, Anthropic is an AI safety and research company. It is focused on developing large-scale AI systems that are helpful, honest, and harmless, with a strong emphasis on ethical alignment with human values. Website: https://anthropic.com/ Key Product: Claude is a family of large language models designed to be a safe, helpful, and harmless AI assistant for a wide range of conversational and text-processing tasks.
    

28. Phenom Bio: Phenom is an AI-powered HR technology company that helps organizations solve their biggest hiring and retention challenges. Its platform connects data from all talent interactions to create a more personalized and efficient experience for candidates and employees. Website:https://www.phenom.com/ Key Product: Phenom offers a suite of talent acquisition and management platforms that automate and personalize the entire talent journey.
    

29. Scale AI Bio: Scale AI provides essential data infrastructure for training artificial intelligence systems. The company offers tools and services for data annotation, curation, and evaluation, helping organizations build and deploy high-quality AI models. Website: https://scale.com/ Key Product: The Scale Generative AI Platform and Data Engine provide the high-quality data and feedback necessary to build and fine-tune powerful AI applications.
    

30. CentralReach Bio: CentralReach is a software company that provides end-to-end solutions for autism and intellectual and developmental disabilities (IDD) care. Its platform helps therapists and educators manage therapy, practice administration, and clinical data. Website: https://centralreach.com/ Key Product: CentralReach offers an ABA (Applied Behavior Analysis) and multidisciplinary therapy platform that streamlines workflows for clinicians and care providers.
    

31. Glean Bio: Glean is an AI-powered enterprise search and knowledge discovery platform. It uses natural language processing to help employees find and summarize information instantly across all of a company's applications and internal documents. Website: https://www.glean.com/ Key Product:Glean's platform provides personalized knowledge discovery and a unified search experience to help employees access company information efficiently.
    

32. LinkSquares Bio: LinkSquares provides an AI-powered platform for contract lifecycle management (CLM). It helps legal and business teams draft, review, and analyze contracts more efficiently by providing AI-driven analytics and automated workflows. Website: https://www.linksquares.com/ Key Product: LinkAI is the company's suite of predictive and generative AI tools designed specifically for managing and extracting insights from legal contracts.
    

33. Prezent Bio: Prezent is an AI platform designed to improve business communications by automating the creation of professional presentations. It uses contextual intelligence to help users build compelling narratives and on-brand slides quickly. Website: https://www.prezent.ai/ Key Product: Prezent provides an enterprise presentation and narrative tool that helps standardize and accelerate the creation of business communications.
    

34. Adept Bio: Adept is an AI research and product lab building general intelligence. The company focuses on creating AI agents that can act as collaborators, automating complex software workflows by understanding and executing natural language commands. Website: https://www.adept.ai/ Key Product: Adept is developing AI agents that can perform complex, multi-step tasks within existing software applications, enabling seamless human-machine collaboration.
    

35. Snorkel AI Bio: Spun out of the Stanford AI Lab, Snorkel AI provides a data-centric AI platform for building and managing training data. Its programmatic approach allows enterprises to label and curate data much faster than manual methods, accelerating model development. Website: https://snorkel.ai/ Key Product: The Snorkel Flow platform enables programmatic data labeling and management, allowing teams to build custom AI models more efficiently.
    

36. TensorWave Bio: TensorWave is an AI infrastructure provider that offers a high-performance cloud powered by AMD hardware. It aims to provide a competitive alternative for developers and enterprises seeking powerful computing resources for AI workloads. Website: https://tensorwave.com/ Key Product: TensorWave provides AMD-powered AI cloud infrastructure designed for high-performance computing and large-scale model training.
    

37. SandboxAQ Bio: Spun out from Alphabet, SandboxAQ is a company that combines AI and quantum technology (AQ) to solve complex problems in fields like drug discovery, material science, and security. Website: https://www.sandboxaq.com/ Key Product: AQBioSim is one of its key platforms, leveraging AI and quantum simulation to accelerate the development of new drugs and therapies.
    

38. Runway Bio: Runway is an applied AI research company focused on building the next generation of creative tools for media production. Its platform enables artists and content creators to generate and edit video, images, and other media using AI models. Website: https://runwayml.com/ Key Product:The Gen-3 Alpha video model is Runway's latest text-to-video generation tool, allowing for the creation of highly realistic and controllable video content.
    

39. OpenAI Bio: OpenAI is a leading AI research and deployment company with a mission to ensure that artificial general intelligence benefits all of humanity. It is renowned for developing some of the world's most advanced large language models. Website: https://openai.com/ Key Product: ChatGPT and the GPT series of models, accessible via API, are powerful large language models that can understand and generate human-like text.
    

40. Insilico Medicine Bio: Insilico Medicine is a clinical-stage biotech company that uses generative AI for drug discovery and development. Its platform accelerates the entire process, from identifying novel targets to designing new molecules and predicting clinical trial outcomes. Website: https://insilico.com/ Key Product: The Pharma.AI platform is an end-to-end generative AI solution for discovering and developing new drugs.
    

41. Lila Sciences Bio: Lila Sciences is an ambitious startup building superintelligence platforms with the goal of accelerating scientific discovery. The company is focused on creating AI systems that can reason and collaborate with scientists to solve major research challenges. Website: https://www.lila.ai/ Key Product: The company is developing advanced AI for research acceleration, aiming to build systems that can function as scientific partners.
    

42. Reflection.Ai Bio: Reflection.Ai is a research and product company dedicated to developing autonomous, superintelligent systems. Its work focuses on creating agentic AI frameworks that can reason, plan, and execute complex tasks independently. Website: https://reflection.ai/ Key Product:The company is building agentic AI frameworks as the foundation for developing autonomous and highly capable intelligent systems.
    

    
    

43. Capacity Bio: Capacity has developed a fully AI-powered helpdesk designed to automate support and business processes. The platform learns from employee and customer interactions to answer questions, complete tasks, and improve overall satisfaction and efficiency. Website: https://capacity.com/ Key Product: The Capacity platform is an AI-powered helpdesk that automates workflows and provides instant support across an organization.
    

44. Read AI Bio: Read AI uses artificial intelligence, natural language processing (NLP), and computer vision to measure and analyze engagement in online meetings. The platform provides real-time dashboards, summaries, and analytics to help make meetings more effective. Website:https://www.read.ai/ Key Product: Read AI is a meeting analytics tool and scheduler that provides insights into participant engagement, sentiment, and key moments.
    

45. Plaud AI

    Bio: Plaud AI develops wearable technology for automated meeting and conversation documentation. Its AI-powered voice recorders transcribe discussions, generate summaries, and support multiple devices and languages for seamless note-taking.

    
    

    Website: https://plaud.ai Key Product: The Plaud Note is a wearable AI voice recorder that offers high-quality transcription and summarization for meetings and calls.
    

46. Deepgram Bio: Deepgram provides a high-performance speech recognition platform for developers. The company specializes in delivering fast, accurate, and scalable transcription and analysis of audio and video content across multiple languages.

    Website: https://deepgram.com/

    Key Product: The Deepgram Aura platform is an AI speech recognition and understanding solution offered via API for real-time and pre-recorded audio.
    

47. Arthur AI Bio: Arthur AI is an AI performance company that helps enterprises monitor, measure, and improve their machine learning models. It provides essential tools for detecting biases, ensuring fairness, and protecting large language models from risks.

    
    

    Website: https://www.arthur.ai/

    Key Product: Arthur Shield is a firewall for large language models (LLMs) that detects and prevents hallucinations, data leakage, and other vulnerabilities.
    

48. Spot AI Bio: Spot AI leverages artificial intelligence to analyze consumer behavior and enhance customer engagement. The platform delivers personalized recommendations and real-time marketing analytics to help businesses understand and connect with their audience. Website: https://www.spot.ai/Key Product: The company provides an AI-driven customer engagement analytics platform for delivering personalized user experiences.
    

49. Fireflies.ai Bio: Fireflies.ai is an AI meeting assistant that integrates with major video-conferencing platforms like Zoom and Google Meet. It automatically records, transcribes, summarizes, and analyzes voice conversations to make meetings more productive. Website: https://fireflies.ai Key Product: Fred is the AI meeting assistant from Fireflies.ai that captures and organizes all key information from meetings.
    

50. Otter AI Bio: Otter AI is a well-known speech-to-text service that provides real-time transcription for meetings, interviews, and events. Its AI technology creates automated notes, identifies speakers, and generates action items from audio and video. Website: https://otter.ai/ Key Product: OtterPilot™ is an AI meeting assistant that automatically joins meetings, captures notes, and shares summaries to keep teams aligned.
    

51. Educato AI Bio: An early-stage Y Combinator startup, Educato AI focuses on creating AI-powered exam preparation platforms. It targets professional and academic exams that currently lack high-quality online study materials, generating content to fill the gap. Website: https://www.educato.ai Key Product:Educato AI provides AI-generated exam preparation content and platforms for specialized tests.
    

52. Avelis Health Bio: Avelis Health is a pre-seed Y Combinator startup using AI to enhance payment integrity in healthcare. Its platform audits medical claims to identify and reduce improper payments for employers and health plans. Website: https://www.avelishealth.com Key Product: The company offers an AI-powered payment integrity solution for auditing and correcting medical claims.
    

53. CreativeMode Bio: A Y Combinator-backed startup, CreativeMode is building a platform that enables no-code mod creation for popular games like Minecraft. It uses AI to allow gamers to design and implement their own game modifications without programming skills. Website: Not available Key Product: CreativeMode is developing an AI-powered game modification tool that allows for no-code content creation.
    

54. StarSling Bio: StarSling, a pre-seed Y Combinator startup, is building an AI agentic developer homepage. This tool is designed to automate common software development tasks such as deployments and bug fixes, streamlining the workflow for engineers. Website: Not available Key Product: StarSling provides an AI developer automation tool that acts as an agentic homepage for engineers.
    

55. Shepherd Bio: Shepherd is an early-stage Y Combinator startup creating an AI-enhanced learning assistant for schools. The platform combines AI-driven self-study modules, personalized tutoring, and performance analytics to support student learning. Website: Not available Key Product: Shepherd offers an AI learning assistant that integrates self-study, tutoring, and analytics for a comprehensive educational experience.
    

56. Domu Technology Inc. Bio: Backed by Y Combinator, Domu Technology Inc. is developing an AI solution to automate debt collection. Its system is designed to handle collection calls for banks and insurance companies, improving efficiency and consistency. Website: Not available Key Product:Domu provides an AI-powered debt collection automation service for financial institutions.
    

57. Clearest Health Bio: Clearest Health is a pre-seed Y Combinator startup rebuilding health insurance infrastructure from the ground up using AI. Its platform focuses on improving care navigation and simplifying the insurance experience for members. Website: Not available Key Product: The company is building an AI-native health insurance platform to facilitate better care navigation and administration.
    

58. Ora AI Bio: An early-stage Y Combinator startup, Ora AI is focused on providing AI-native education. Its initial product targets medical school training, using AI to create more effective and personalized learning experiences for future doctors. Website: Not available Key Product: Ora AI is developing an AI medical education platform to modernize training for medical students.
    

59. Sohar Health Bio: Sohar Health, a Y Combinator startup, offers an AI-driven solution for healthcare revenue cycle management (RCM). The platform focuses on insurance verification to ensure claim accuracy and reduce administrative burdens for providers. Website: Not available Key Product: Sohar Health provides an AI-powered RCM platform for accurate and automated healthcare insurance verification.

    
    

60. Rove

    Bio: Rove is a pre-seed Y Combinator startup creating a universal airline mile program. Using AI, it aims to develop a single, redeemable rewards currency that can be used across different airlines and hotels, simplifying travel loyalty. Website: Not available Key Product: Rove is building an AI-powered universal rewards program for seamless redemption of travel miles.
    

61. Corgi

    Bio: An early-stage Y Combinator company, Corgi is building a full-stack AI insurance carrier. The startup is targeting the US insurance market with a technology-first approach to streamline underwriting, claims, and customer service. Website: Not available Key Product: Corgi is developing an AI-native insurance platform to operate as a modern, technology-driven carrier.
    

62. Pointhound

    Bio: Backed by Y Combinator, Pointhound uses AI to simplify the process of redeeming credit card points for flights. Its tool helps users find the best value for their points, navigating complex loyalty programs automatically. Website: Not available Key Product: Pointhound offers an AI-powered points redemption tool that optimizes the use of credit card rewards for travel.
    

63. Ocular AI Bio: Ocular AI is a pre-seed Y Combinator startup specializing in data annotation for artificial intelligence. Its engine provides high-quality data labeling services for generative AI and computer vision models. Website: Not available Key Product: The Ocular AI engine is a platform for advanced data labeling and annotation for AI model training.
    

64. Pernell Bio: Pernell, an early-stage Y Combinator startup, offers an AI-powered second phone number service. The service is designed for call recording, transcription, and summarization, providing a smart assistant for voice communications. Website: Not available Key Product: Pernell provides an AI-powered second phone line for automated call transcription and summarization.
    

65. Displaid Bio: A spin-off from MIT, Displaid develops technology to monitor the structural health of bridges. It uses a combination of wireless sensors and AI to analyze infrastructure integrity and provide predictive maintenance insights. Website: https://www.displaid.it/en/ Key Product: Displaid offers an AI-driven bridge monitoring system that uses sensor data to ensure structural safety.
    

66. Eva Bio: Eva is an MIT-affiliated startup that develops digital twin technology to accelerate AI model training. By leveraging advanced semiconductor technology, it creates virtual replicas to speed up development cycles and reduce costs. Website: https://evacorp.ai/about/ Key Product: Eva creates digital twins of semiconductor systems to significantly shorten the training time for AI models.
    

67. Gaia AI Bio: An MIT spin-off, Gaia AI uses artificial intelligence and satellite imagery to map and measure forests. Its technology provides faster and more accurate data for carbon offsetting, timber management, and wildfire prevention. Website: https://www.gaia-ai.eco/ Key Product: Gaia AI develops forest analysis algorithms that process satellite and aerial imagery for ecological and commercial insights.
    

68. GetIntro Bio: GetIntro is an MIT-affiliated startup that uses AI agents to facilitate sales introductions within healthcare networks. Its platform automates the process of connecting sales teams with key decision-makers in the medical industry. Website: https://www.getintro.com/ Key Product: GetIntro provides an AI sales agent specifically designed for generating introductions and leads in the healthcare sector.
    

69. Perygee Bio: A spin-off from MIT, Perygee builds software that integrates disparate enterprise systems using AI. Its platform helps organizations streamline workflows and improve operational efficiency by connecting their existing software tools. Website: https://perygee.com/ Key Product: Perygee offers AI-powered enterprise integration software that unifies and automates workflows across different systems.
    

70. SafeMode Bio: SafeMode is an MIT-affiliated startup that uses AI to improve driver safety in the trucking industry. Its program analyzes and modifies driver behavior to reduce accidents, improve fuel efficiency, and lower insurance costs. Website: https://www.safemode.co/ Key Product: The company provides an AI-driven driver behavior program designed to create safer and more efficient commercial fleets.
    

71. Tinfoil Bio: An MIT spin-off, Tinfoil is focused on securing confidential AI applications in the cloud. It uses advanced encryption and confidential computing techniques to protect sensitive data while it is being processed by AI models. Website: https://tinfoil.sh/ Key Product: Tinfoil offers a confidential computing platform that enables secure processing of sensitive data for AI applications.
    

72. Tristar AI Bio: Tristar AI is an MIT-affiliated startup that develops AI-powered vision systems for manufacturing inspection. Its technology monitors production lines in real-time to detect defects and send alerts, improving quality control. Website: https://www.tristar.ai/ Key Product: Tristar AI provides AI-powered manufacturing inspection systems that use computer vision to automate quality assurance.
    

73. Unbox AI Bio: A spin-off from MIT, Unbox AI has developed a specialized large language model called BehaviorGPT. This model is designed to predict consumer behavior by analyzing various data sources, providing valuable insights for businesses. Website: https://unboxai.com/ Key Product: BehaviorGPT is a proprietary LLM that predicts consumer behavior and market trends.
    

74. Vocadian Bio: Vocadian is an MIT-affiliated startup that manages workforce fatigue using AI. Its system analyzes voice biomarkers to assess fatigue levels, helping organizations in high-risk industries prevent accidents and improve employee well-being. Website: https://www.vocadian.ai/ Key Product: The company offers an AI-powered fatigue risk management system that uses vocal analysis to monitor workforce alertness.
    

75. Aaru Bio: Aaru is a startup that creates large-scale population simulations for complex decision-making. Its technology generates synthetic data for applications like consumer behavior modeling and policy impact analysis. Website: https://app.cbinsights.com/profiles/c/Z5zr4 Key Product: Aaru develops synthetic data simulation platforms for modeling complex systems and predicting outcomes.
    

76. Unstructured Bio: Unstructured develops tools to process complex documents like PDFs, Word files, and presentations. Its platform captures and transforms data from these sources into AI-friendly formats, making it ready for use in machine learning models. Website:https://www.cbinsights.com/company/unstructured Key Product: Unstructured provides data preparation and pre-processing tools that convert enterprise documents into formats suitable for LLMs.
    

77. Qdrant Bio: Qdrant is the company behind the popular open-source vector database of the same name. Its technology provides the essential infrastructure for building AI applications that require high-performance similarity search, such as recommenders and semantic search. Website:https://www.cbinsights.com/company/qdrant Key Product: Qdrant is a high-performance, open-source vector database and search engine for AI applications.
    

78. Archetype AI Bio: Archetype AI is building foundation models that understand the physical world. Its technology processes multimodal sensor data—from video to radio frequencies—to provide real-time insights about physical environments and events. Website:https://www.cbinsights.com/company/archetype-ai Key Product: Newton is the company's multimodal AI model designed to interpret complex sensor data from the physical world.
    

79. LangChain Bio: LangChain is a company that develops frameworks for building context-aware and reasoning-based AI applications. Its open-source tools help developers chain together language models with other data sources and APIs for more complex tasks. Website:https://www.cbinsights.com/company/langchain Key Product: LangChain provides an AI agent orchestration framework for building, debugging, and deploying sophisticated LLM-powered applications.
    

80. Arize Bio: Arize provides an AI observability platform that helps teams monitor the performance of machine learning models in production. Its tools are used to detect and troubleshoot issues like data drift, performance degradation, and bias. Website: https://www.cbinsights.com/company/arize-ai Key Product: The Arize AI platform offers tools for ML monitoring, troubleshooting, and observability to ensure models perform reliably in production.
    

81. Credo AI Bio: Credo AI offers a platform for AI governance, risk management, and compliance. Its software helps organizations operationalize responsible AI principles by automating audits, policy enforcement, and reporting. Website: https://www.cbinsights.com/company/credo-ai Key Product:Credo AI provides an AI governance platform that helps organizations ensure their AI systems are fair, transparent, and compliant.
    

82. Zama Bio: Zama is a cryptography company that develops technology for privacy-preserving machine learning. Its tools enable computation on encrypted data, allowing AI models to be trained and run without exposing sensitive information. Website: https://www.cbinsights.com/company/zama Key Product: Zama offers open-source fully homomorphic encryption (FHE) solutions for secure machine learning on encrypted data.
    

83. EdgeRunner AI Bio: EdgeRunner AI focuses on creating efficient AI models that can run on local devices. It uses an ensemble of task-specific models to solve problems without relying solely on large, cloud-based general-purpose models. Website: Not available Key Product: The company develops ensemble AI models designed for efficient, on-device problem-solving.
    

84. LMArena Bio: LMArena provides a platform for community-driven benchmarking of AI models. It allows users to compare the performance of different large language models in a crowdsourced, transparent manner. Website: Not available Key Product: LMArena is an AI model benchmarking tool that offers community-driven leaderboards and performance comparisons.
    

85. Nexthop AI Bio: Nexthop AI is a startup focused on delivering next-generation AI infrastructure solutions. The company aims to provide the high-performance computing resources and platforms needed to support advanced AI development. Website: https://nexthop.ai/news-and-event/press-release-company-launch/ Key Product: Nexthop AI provides specialized AI infrastructure designed to accelerate the development and deployment of large-scale models.
    

86. Turing Bio: Turing collaborates with leading large language model companies to develop and implement advanced AI coding solutions. The company integrates generative AI into the software development lifecycle to enhance developer productivity. Website: Not available Key Product: Turing provides AI-powered coding solutions and developer tools in partnership with major LLM providers.
    

87. Roboflow

    Bio: Roboflow offers a comprehensive platform that simplifies the process of building computer vision models. It provides tools for data labeling, dataset management, model training, and deployment, making computer vision more accessible.
    

    Website: https://roboflow.com

    Key Product: The Roboflow platform is an end-to-end solution for managing datasets and training computer vision models.
    

88. DevRev

    Bio: DevRev is a company focused on bringing developers closer to their customers. Its platform uses conversational AI and data analytics to connect product development, support, and customer feedback into a single, unified experience.

    Website: https://devrev.ai

    Key Product: DevRev provides a conversational AI platform that integrates customer relationship management (CRM) with product development workflows.
    

89. People.ai

    Bio: People.ai provides an enterprise revenue intelligence platform that uses AI to analyze customer interaction data. It captures sales, marketing, and customer success activity to deliver insights that help businesses increase revenue.
    

    Website: https://www.people.ai

    Key Product: The People.ai enterprise revenue platform uses AI to analyze sales data and provide actionable insights for revenue teams.
    

90. Abnormal Security

    Bio: Abnormal Security is a cybersecurity company that uses AI to protect organizations from advanced email attacks. Its platform analyzes behavior and content to detect threats like business email compromise, phishing, and malware.
    

    Website: https://abnormalsecurity.com/

    Key Product: The Abnormal Integrated Cloud Email Security (ICES) platform uses behavioral AI to detect and block sophisticated email attacks.
    

91. Latent Labs

    Bio: Founded by alumni from DeepMind, Latent Labs is a biotech company building AI foundation models for biology. Its goal is to make biology programmable, partnering with other biotech firms to accelerate the development of new therapeutics.
    

    Website: https://latentlabs.ai

    Key Product: Latent Labs develops AI foundation models for proteins and other biological systems to advance drug discovery and biotechnology.
    

92. AcuityMD Bio: A Boston-based company recognized by Forbes as a "Next Billion-Dollar Startup" for 2025. AcuityMD has built a commercial intelligence platform specifically for the medical technology industry, helping device manufacturers target their products and clinical trials more effectively. Website: https://www.acuitymd.com/ Key Product: A medtech commercial intelligence platform that analyzes de-identified data for over 325 million people. It allows medtech companies to identify the right physicians and patients for their products and trials.
    

93. PathAI Bio: A company operating at the intersection of software and medicine, PathAI is on a mission to improve patient outcomes using AI-powered pathology. It collaborates with biopharmaceutical companies and clinical laboratories to accelerate drug development and improve diagnostic accuracy. Website: https://www.pathai.com/ Key Product: The AISight® Digital Pathology Platform is a cloud-native solution for managing and analyzing digital pathology images, complemented by AI algorithms like TumorDetect that assist pathologists in making more precise cancer diagnoses.
    

94. Anduril Industries Bio: A unicorn defense technology company founded with a Silicon Valley ethos to transform U.S. and allied military capabilities. Anduril develops advanced hardware and software products built around AI and autonomous systems to solve critical national security challenges. Website: https://www.anduril.com/ Key Product:The Lattice OS (Operating System) is the AI-powered software backbone that connects and controls Anduril's entire family of hardware, including autonomous aircraft, sentry towers, and underwater vehicles, into an integrated defense network.
    

95. Palantir Technologies Bio: A publicly traded software company specializing in big data analytics, Palantir is best known for its work with government, defense, and intelligence agencies. It helps organizations integrate and analyze vast, disparate datasets to uncover critical insights and patterns. Website: https://www.palantir.com/ Key Product:Palantir's two main platforms are Gotham and Foundry. Gotham is designed for the government sector to identify threats, while Foundry creates a central operating system for commercial enterprises to optimize their data.
    

96. Waymo Bio: Originally the Google Self-Driving Car Project, Waymo is a subsidiary of Alphabet and a global leader in autonomous driving technology. With a mission to be the "world's most trusted driver," the company operates the first and largest fully autonomous ride-hailing service. Website: https://waymo.com/ Key Product: The Waymo Driver is the company's integrated autonomous driving technology, which combines lidar, cameras, and radar with powerful AI software. This system powers a fleet of all-electric vehicles providing 24/7 ride-hailing services in major US cities.
    

97. Zipline Bio: A global leader in autonomous logistics, Zipline designs, manufactures, and operates the world's largest automated on-demand delivery system. The company has completed over one million autonomous deliveries of medical supplies, vaccines, and consumer packages. Website: https://www.flyzipline.com/ Key Product: An end-to-end autonomous drone delivery system that includes a fleet of fixed-wing drones, automated launch and recovery infrastructure, and a logistics network that enables precise, parachute-based package drops.
    

98. Midjourney Bio: An independent, self-funded research lab focused on exploring new mediums of thought and expanding the imaginative powers of the human species. Midjourney has gained widespread acclaim for its ability to produce uniquely artistic and high-fidelity images from text prompts. Website: https://www.midjourney.com/ Key Product: A generative AI image service that allows users to create complex, detailed, and aesthetically compelling visuals using natural language descriptions, primarily accessed through a bot on the Discord platform.
    

99. Anterior Bio: Formerly known as Co:Helm, Anterior is a New York-based startup backed by Sequoia Capital and NEA. The company is building an AI co-pilot to reduce the administrative burden on healthcare professionals, starting with health insurance prior authorizations. Website: https://www.anterior.com/ Key Product: An AI platform that leverages LLMs to automate the collection and analysis of medical documentation required to get procedures approved by insurance companies, freeing up nurses and doctors from time-consuming paperwork.
    

100. Harmonic Bio: A highly ambitious and well-funded startup operating in stealth mode. Backed by $100 million from premier venture firms like Sequoia and Kleiner Perkins, Harmonic is reportedly focused on the grand challenge of building "mathematical superintelligence." Website: https://www.harmonic.sh/ Key Product: The company is developing foundational AI models with a focus on advanced reasoning and mathematical capabilities, aiming to achieve breakthroughs in artificial general intelligence (AGI).

FAQs and Answers

What are the top AI startups in the USA for 2025?

 The 2025 AI 100 features top startups like Anysphere (Cursor coding tool), Mercor (AI hiring platform), and World Labs (spatial intelligence models). This definitive directory covers 100 innovative companies across sectors like healthcare, robotics, and infrastructure, updated as of September 2025.

How can I find a list of 2025 AI startups in the USA? 

Check out "The 2025 AI 100: The Definitive Directory," which lists 100 USA-based AI startups. It includes names, bios, websites, and key products, sourced from 2025 industry reports. Visit the blog under the "AI Startup Insights" category for the full directory.

Which AI startups are worth investing in for 2025?

 Promising 2025 AI startups include Thinking Machine Labs ($9B valuation), Together AI ($3.3B), and OpenEvidence (unicorn status). The 2025 AI 100 directory highlights investment potential across pre-seed to unicorn stages—review their products and trends for informed decisions.

What new AI startups emerged in the USA in 2025?

 New 2025 entrants include Thinking Machine Labs (founded by Mira Murati), World Labs (by Fei-Fei Li), and CreativeMode (YC-backed game AI). The 2025 AI 100 directory tracks these and other emerging USA startups, offering insights into their innovations.

Where are the best AI startups located in the USA for 2025?

Top locations include San Francisco (Anysphere, Together AI), New York (Frame AI), and Boston (Insilico Medicine). The 2025 AI 100 directory maps 100 startups by region, showcasing hubs driving AI growth across the USA.

How often is the 2025 AI 100 directory updated?

The 2025 AI News hub's AI 100 directory is updated as of September 2025 and will be refreshed periodically to reflect new startups and developments. Check the "AI Startup Insights" category for the latest version.

5. Conclusion: Beyond the Hype, a New Foundation Is Being Built

Navigating this list of 100 startups reveals a clear and powerful narrative for 2025: AI is maturing. The initial wave of hype around general-purpose models has given way to a more pragmatic and potent phase of innovation. The most compelling companies featured here are not just building tools; they are deploying intelligent, autonomous agents deep within the critical workflows of every major industry.

From Anterior automating healthcare bureaucracy to Mandel AI creating a self-aware supply chain, the story of 2025 is that AI is no longer a feature—it's becoming the foundational layer for the next generation of technology and business. The startups on this list are not just participants in this revolution; they are its architects.

As this incredible pace of innovation continues, the companies that succeed will be those that can seamlessly integrate intelligence into our daily lives and workflows, solving real problems and creating undeniable value. The future is being coded, trained, and deployed right now, in the labs and offices of the companies you've just explored.

What emerging AI trend are you most excited about? Share your thoughts with A.I News Hub in the comments below!